Description:
2 x SYBR Green qPCR Mix provides rapid real-time quantification of RNA targets in SYBR Green fluorescent. The components of 2 x SYBR Green qPCR Mix include Hot-start enzyme named HotMaster Taq DNA Polymerase, dNTP, PCR reaction buffer, BSA and SYBR Green , and so on. The fluorescent dye SYBR Green I in the master mix enables the analysis of many different targets without having to synthesize target specific labeled probes. High specificity and sensitivity in PCR are achieved by the use of the hot-start enzyme HotMaster Taq DNA Polymerase together with a specialized PCR buffer. Unlike other “hot-start” Taq DNA polymerase formulations that block the enzyme activity only in the first high temperature step, HotMaster Taq DNA inhibitors closes the polymerase-substrate binding site by temperature adjustment method. Inactive polymerase-inhibitor complexes are formed at temperatures below 40°C. As the temperature is elevated to the primer-specific annealing temperature, the binding equilibrium is shifted towards complex formation only with target-specific primed template DNA. This prevents the formation of misprimed products and primer–dimers during reaction setup and the first denaturation step, leading to high PCR specificity and accurate quantification.
