Description:
Cells are lysed during a short incubation with Proteinase K in the presence of a chaotropic salt (guanidine-HCl), which immediately inactivates all nucleases. Cellular nucleic acids (NA) bind selectively to special silicon membrane pre-packed in the spin columns. Bound NA is purified in a series of rapid "wash-and-spin" steps to remove contaminating cellular components. A special Inhibitor Removal Buffer (Buffer IR) has been included which allows even the application of heparinized sample material with 100 U/ml of Heparin. Finally, low salt elution releases the NA from the silicon membrane. This simple method eliminates the need for organic solvent extractions and DNA precipitation, allowing for rapid purification of many samples simultaneously.
