Description:
Plant DNA mini Kit provide a fast and easy way to purify DNA from plant and fungaltissue. Up to 100 mg of tissue can be processed. Easy-to-use procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and Southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. This makes the kits highly suited for simultaneous processing of multiple samples.
Purified DNA is eluted in low-salt buffer or water, ready for use in downstream applications. DNA purified using the kits is up to 40 kb in size, with fragments of 20–25 kb predominating. DNA of this length denatures completely in PCR and shows the highest amplification efficiency. Purified DNA has an A260/A280 ratio of 1.7–1.9, indicating high purity of the DNA.
The column membrane ensures complete removal of all inhibitors of PCR and other enzymatic reactions. DNA purified using the kits is highly suited for use in all downstream applications, including PCR, RAPD analysis, AFLP analysis, RFLP analysis, Southern blotting, microsatellite analysis, SNP genotyping, and quantitative,real-time PCR. .
